Metabolite profile of tree marigold (Tithonia diversifolia (Hamsley) A. Grey) leaves

Tree marigold or Tithonia diversifolia (Hemsley) A. Grey) also known as Insulin leaves has traditionally been used as an herb for the treatment of diabetes mellitus (DM). Tree marigolds contain various metabolite compounds, which have various bioactivities, so research was carried out to study metabolite profiles using a non-target metabolism approach using a GC-MS (Gas Chromatography-Mass Spectrometry) instrument. The results of GC-MS analysis of Tree marigold ethanol extract showed 30 peaks in the chromatogram, of which 7 were dominant metabolite compounds including 1-butanol, 3-methyl acetate (28.11%), Thunbergol (14.28%), Geranyl acetate (12.13%). ), D-Limonene (6.64%), 4H-Pyran-4-one, 2,3-Dihydro-3,5-dihydroxy-6 -methyl (5.05%), acetic acid (4.10%), 7.8-epoxy-α-ionone (2.91%). The various categories of metabolites consist of the monoterpene class followed by fatty acids, terpenes, and sesquiterpenoids, as well as other compounds in the terpenoid esters class, alkanoic acids, fatty acids, proteins, amino acids, furanoids, ketones, and others. These metabolite compounds have antidiabetic, antioxidant, anti-inflammatory, antimicrobial, antibacterial, anticancer, and antitumor effects so they have potential as antidiabetic therapy.