Antibacterial effect of Jaft extract and molecular study of cnf-1genes in Uropathogenic Escherichia coli

Background: Escherichia coli is one of the more causative and frequent pathogens of urinary tract infection.

Objectives: The study conducted to evaluate the potential of Inner Stratum of Oak fruit (Jaft) extract in uropathogenic E.coli. Determine the effectiveness of an antibiotic against uropathogenic E.coli and detecting the genes codes of cytotoxic necrotizing factor1 (cnf-1) by PCR technique.

 Methods: Four clinical samples of E.coli were gained from patients with urinary tract infections. Diagnosis of isolates was draw on conventional bacteriological methods and final identification was involved by vitek 2 system. Four concentration (100%, 75%, 50%, 25% mg/ml) from ethanolic extract of Jaft was prepared and used agar wells diffusion method to execution antibacterial activity. Kirby -Bauer method was utilized to assess six of an antibiotics against isolates. Polymerase chain reaction technique was used in investigation of cytotoxic necrotizing factor 1 (cnf-1) genes in E.coli.

Results: The results of antibiotics sensitivity test showed that all four isolates of E.coli were highly sensitive (100%) to Meropenem, Amikacin and resistance (100%) to Trimethoprim, whearse (75%) of isolates were resistance to Ceftriaxone, Ceftazidim, and Levofloxacin. The results of evaluation of plant extract appear that Jaft extract inhibited E.coli in all concentrations and the maximum of inhibition zones were in concentration (50% mg/ml) and diameters of inhibition zones were (20 mm in three isolates and 27mm in last one). Cytotoxic necrotizing factor1 (cnf-1) genes were found out by molecular technique in four isolates, the results revealed that all isolates (100%) of E.coli were carried this gene.

 Conclusion: We found in this study that UTI is caused by multi drug resistance (MDR) E.coli also found that the antibacterial effect of ethanolic extract of Jaft was strong against uropathogenic E.coli and cnf-1 genes present in all isolates ,this indicate Cytotoxic necrotizing factor 1 play an important role in infectious disease such as UTI .